<i>Math1</i>Gene Transfer Generates New Cochlear Hair Cells in Mature Guinea Pigs<i>In Vivo</i>

TLDR

Hair cell loss in the mammalian cochlea is irreversible, and Math1—a transcription factor that drives hair cell differentiation during development—is expressed in developing hair cells but not in nonsensory cells. The study aimed to determine the effect of overexpressing Math1 in nonsensory cochlear cells. In vivo delivery of an adenovirus carrying Math1 into the endolymph of mature guinea pig cochleae caused Math1 overexpression in supporting cells and adjacent nonsensory epithelial cells. This overexpression produced immature hair cells in the organ of Corti and new hair cells in adjacent regions, with auditory nerve axons extending toward some of these cells, showing that mature nonsensory cells can generate new hair cells when Math1 is overexpressed.

Abstract

Hair cell loss in the mammalian cochlea is irreversible and results in permanent hearing loss. Math1, the basic helix-loop-helix transcription factor homolog of the Drosophila atonal gene, is a positive regulator of hair cell differentiation during cochlear development. Developing hair cells express Math1, and nonsensory cells do not. We set out to determine the outcome of overexpression of Math1 in nonsensory cells of the cochlea on the phenotype of these cells. We demonstrate that in vivo inoculation of adenovirus with the Math1 gene insert into the endolymph of the mature guinea pig cochlea results in Math1 overexpression in nonsensory cochlear cells, as evident from the presence of Math1 protein in supporting cells of the organ of Corti and in adjacent nonsensory epithelial cells. Math1 overexpression leads to the appearance of immature hair cells in the organ of Corti and new hair cells adjacent to the organ of Corti in the interdental cell, inner sulcus, and Hensen cell regions. Axons are extended from the bundle of auditory nerve toward some of the new hair cells, suggesting that the new cells attract auditory neurons. We conclude that nonsensory cells in the mature cochlea retain the competence to generate new hair cells after overexpression of Math1 in vivo and that Math1 is necessary and sufficient to direct hair cell differentiation in these mature nonsensory cells.