Annual Review of Biophysics and Bioengineering · 1982 · 28 citations · 0 references
Life SciencesGeneticsMolecular BiologyGenetic EngineeringGenome EditingMicrobial GeneticsCrispr-based EngineeringCrispr-cas SystemsGuide Rna DesignPlasmid BiologyPlasmid GeneticsTarget Site SelectionBacterial Crispr DefenseConjugative ElementsConjugative PlasmidsConjugative TransferCrispr-cas9 Genome EditingDual Guide Rna PairingEndonuclease MechanismsX-ray Computed Tomography
Many bacterial clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated (Cas) systems employ the dual RNA–guided DNA endonuclease Cas9 to defend against invading phages and conjugative plasmids by introducing site-specific ...Read More