Solid‐Phase Oligosaccharide Tagging (SPOT): Validation on Glycolipid‐Derived Structures

Abstract

SPOT check: A simple technique for labeling oligosaccharides consists of capturing the sugars on beads functionalized with hydroxylamine groups bearing cleavable linkers. After sequential pipetting in and out of reagents, tagged sugars are released into solution (see picture; PEGA=polyethylene glycol–polyacrylamide resin) for analysis by electrophoresis or mass spectrometry.