Tissue Antigens · 1995 · 22 citations · 33 references
Life SciencesGeneticsMolecular GeneticsHuman GeneticsImmunogeneticsRheumatologyAnkylosing SpondylitisAutoimmune Disease Susceptibility GenesAutoimmune DiseasesDisease Susceptibility LociGenetic Susceptibility To AutoimmunityHla TypingAllelic Frequency VariationAllelic Variation AnalysisHla Allele Frequency DistributionHla Allele PolymorphismHla AllelesHla Genotype AssociationsHla Polymorphism AnalysisHuman Leukocyte Antigen B27
The objective was to determine the role of the TAP 1 gene in influencing the phenotype of disease in adult patients with ankylosing spondylitis (AS). The distribution of TAP 1 gene alleles was determined using the PCR RFLP method and restriction enzymes Bcl I and Acc I. The study population included 115 HLA-B27 positive patients with well-documented AS and 41 HLA-B27 positive normal controls. No significant difference in distribution of TAP 1 alleles was noted in comparisons of all AS patients with normal controls. However, AS patients with extraspinal disease were noted to have a significantly increased prevalence of the TAP 1B allele (17.0%) as compared to AS patients without extraspinal disease (2.9%) (P = 0.005) or normal controls (1.9%) (P = 0.005). Polymorphism at the TAP 1 locus may influence disease outcome in patients with AS.
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A fast method for high-quality genomic DNA extraction from whole human blood.
Stefano Gustincich, Guidalberto Manfioletti, Giannino Del Sal et al. · PubMed · 1991 · 606 citations