Nucleic Acids Research · 2003 · 20 citations · 42 references
Life SciencesBiochemistryMolecular BiologyComparative GenomicsEnzymologyMicrobial GeneticsEnzyme SpecificityEnzyme StructureEnzyme-substrate RecognitionRestriction Enzyme TechnologySubstrate SpecificityProtein Recognition SpecificityRecognition Site SpecificityRestriction Endonuclease SpecificityRestriction EndonucleasesRestriction Enzyme ClassificationRestriction Enzyme PurificationType Ii Restriction EnzymesType Iis Restriction Enzymes
The TspDTI restriction endonuclease, which shows a novel recognition specificity 5'-ATGAA(N(11/9))-3', was isolated from Thermus sp. DT. TspDTI appears to be a 'twin' of restriction endonuclease TspGWI from Thermus sp. GW, as we have previously reported. TspGWI was isolated from the same location as TspDTI, it recognizes a related sequence 5'-ACGGA(N(11/9))-3' and has conserved cleavage positions. Both enzymes resemble two other class-IIS endonucleases from Thermus sp.: TaqII and Tth111II. N-terminal amino acid sequences of TspGWI tryptic peptides exhibit 88.9-100% similarity to the TaqII sequence. All four enzymes were purified to homogeneity; their polypeptide sizes (114.5-122 kDa) make them the largest class-IIS restriction endonucleases known to date. The existence of a Thermus sp. sub-family of class-IIS restriction endonucleases of a common origin is herein proposed.
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<i>Fok</i> I dimerization is required for DNA cleavage
Jurate Bitinaite, David A. Wah, Aneel K. Aggarwal et al. · Proceedings of the National Academy of Sciences · 1998 · 488 citations · Full text
REBASE: restriction enzymes and methyltransferases
Richard J. Roberts · Nucleic Acids Research · 2003 · 307 citations · Full text