Crystallization and preliminary crystallographic analysis of a 2,3‐dihydroxybiphenyl dioxygenase from <i>Pseudomonas</i> sp. strain KKS102 having polychlorinated biphenyl (PCB)‐degrading activity

Kazuyuki Sugiyama, Hiroki Narita, Takeshi Yamamoto, Toshiya Senda, Kazuhide Kimbara, Norio Inokuchi, Masanori Iwama, Masachika IRIE, Masao Fukuda, Keiji Yano,

Proteins Structure Function and Bioinformatics · 1995 · 14 citations · 11 references

Abstract

Crystals have been obtained for a 2,3-dihydroxybiphenyl dioxygenase (conventionally called BphC) from a polychlorinated biphenyl (PCB)-degrader, Pseudomonas sp. strain KKS102. The crystals were grown using both ammonium sulfate and MPD as the precipitating agents. The crystals belonged to a tetragonal space group (I422) and diffracted to 2.5 A.

References

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