Journal of General Virology · 2011 · 43 citations · 36 references
Dengue VirusGlycobiologyViral PathogenesisMolecular BiologyImmunologyViral Structural ProteinArbovirusGlycosylationSpecific GlycochainBiochemistryVirologyVascular BiologySdc2 Membrane ProteinCell BiologyMolecular VirologyNatural SciencesPathogenesisHeparan SulfateVirus-host InteractionMedicineViral ImmunityCarbohydrate-protein Interaction
Dengue virus (DENV) causes fever and severe haemorrhagic symptoms in humans. The DEN2 16681 strain, derived from a dengue haemorrhagic fever patient, has been widely used in studies related to DENV pathogenesis, such as mouse and non-human primate haemorrhagic models and human vascular endothelial-cell permeability. To clarify the entry mechanism of the 16681 strain, we characterized a novel cell receptor for this strain. Our two major findings were as follows: firstly, the SDC2 membrane protein was an effective DEN2 16681 receptor in a cloned K562 cell line. Secondly, a heparan sulfate (HS) glycochain (of four glycochains in SDC2) is the specific binding site of DENV and seems to be involved in tissue-culture adaptation. Our findings present an entry mechanism that could be implicated for DENV adaptation and HS-mediated DENV infection.
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Erika Navarro-Sánchez, Ralf Altmeyer, Ali Amara et al. · EMBO Reports · 2003 · 493 citations · Full text
Productive Infection, Human Dendritic Cells, Dendritic‐cell‐specific Icam3‐grabbing Non‐integrin +9