Concepedia

Sequence specific interstrand photocrosslinking for effective SNP typing

Takehiro Ami, Katsuya Ito, Yoshinaga Yoshimura, Kenzo Fujimoto

Organic & Biomolecular Chemistry · 2007 · 15 citations · 17 references

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Abstract

We describe a simple and inexpensive SNP typing method by using sequence specific interstrand photocrosslinking via p-carbamoylvinyl phenol nucleosides. Interstrand photocrosslinking showed a high degree of single nucleotide specificity as high as 10(3)-fold and more, and can be used in the diagnostic detection of DNA sequences.

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