Chemical Communications · 2006 · 77 citations · 24 references
Life SciencesBiochemistryBiotechnologyBiocatalysisEnzymologyProtein EngineeringCatalytic MechanismsEnzyme CharacterizationEnzyme FunctionEnzyme StructureEnzyme Surface EngineeringEnzyme-substrate RecognitionEnzyme ActivityEnzyme Activity RegulationEnzyme DimerizationInterfacial Activation Of LipasesLipase CatalysisLipasesSingle-molecule Enzyme AssaysSubstrate Specificity Of Lipases
Click chemistry is used to construct a novel lipase-BSA hetero-dimer, in which the latter protein acts as a foot enabling the anchoring of the enzyme onto the surface for single enzyme studies.
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