PROTEOMICS · 2011 · 241 citations · 32 references
Life SciencesBiochemistryMicrobiologyMedical MicrobiologyMicrobial PathogenesisProteomicsBacterial PathogenesisBacterial Secretion SystemsMass Spectrometry-based ProteomicsMembrane ProteomicsMicrobial ProteomicsProteomic ProfilingExtracellular Vesicle ProteomicsMass Spectrometry-based Membrane ProteomicsMembrane Protein AnalysisOpportunistic Bacterial InfectionsOuter Membrane ProteinsPathogen ProteomicsProteome MiningProteomic Data Analysis
Pseudomonas aeruginosa, an opportunistic human bacterial pathogen, constitutively secretes outer membrane vesicles (OMVs) into the extracellular milieu. Although recent progress has revealed that OMVs are essential for pathogenesis of P. aeruginosa, their proteins have not been comprehensively analyzed so far. In this study, we identified 338 vesicular proteins with high confidence by five separate LC-MS/MS analyses. This global proteome profile provides a basis for future studies to elucidate the pathological functions of OMVs from P. aeruginosa.
32
Universal sample preparation method for proteome analysis
Jacek R. Wiśniewski, Alexandre Zougman, Nagarjuna Nagaraj et al. · Nature Methods · 2009 · 8.7K citations