Rhodobacter capsulatus puc operon: promoter location, transcript sizes and effects of deletions on photosynthetic growth

Abstract

Gene deletions of the puc operon of Rhodobacter capsulatus showed that the pucC and pucE genes, but not pucD, were required for formation of wild-type levels of the LHII complex. Deletion of pucC or pucE also impaired photosynthetic growth. The effects of pucC deletion were suppressed by secondary mutations that mapped outside the puc operon. Fusion of a lac'Z gene to pucE' showed that most of pucE transcription originated from upstream of pucB. RNA blot analysis revealed a 2.4 kb transcript that hybridized to probes specific for the pucBA, pucC and pucDE regions, indicating that some puc operon messages extend from just before the pucB gene to just after the pucE gene.