Concepedia

Minimally Invasive Detection of <i>Piscirickettsia salmonis</i> in Cultivated Salmonids via the PCR

Sergio H. Marshall, Sekou Heath, Vitalia Henríquez, Cristián Orrego

Applied and Environmental Microbiology · 1998 · 67 citations · 15 references

DOIFull text

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Concepts

Abstract

The attributes of the PCR allowed implementation of an assay for specific detection of Piscirickettsia salmonis from a few microliters of fish serum. This opens the way to less invasive modes of sampling for this microbial pathogen in salmonids.

References

15

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Real time quantitative PCR.

Chris Heid, Junko Stevens, Kenneth J. Livak et al. · Genome Research · 1996 · 6.2K citations · Full text

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Chelex 100 as a Medium for Simple Extraction of DNA for PCR-Based Typing from Forensic Material

P. Sean Walsh, David A. Metzger, Russell Higuchi · BioTechniques · 2013 · 5.9K citations · Full text

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Phylogenetic compositions of bacterioplankton from two California estuaries compared by denaturing gradient gel electrophoresis of 16S rDNA fragments

Alison E. Murray, JT Hollibaugh, Cristián Orrego · Applied and Environmental Microbiology · 1996 · 405 citations · Full text

, , +17

Solid-phase reversible immobilization for the isolation of PCR products

Margaret M. DeAngelis, David G. Wang, Trevor Hawkins · Nucleic Acids Research · 1995 · 404 citations

, , +9