FEMS Microbiology Letters · 1996 · 112 citations · 6 references
Veterinary MedicineInfectious DiseasesVeterinary MicrobiologyDiagnostic MicrobiologyInfection PreventionMedical MicrobiologyEgg DisinfectionEnvironmental DisinfectionChemical DisinfectionChemical Egg DisinfectionDisinfectionFluorescent Dyes
In vitro excystation, vital dyes (4', 6-diamidino-2-phenylindole (DAPI) and propidium iodide (PI), and infectivity in neonatal CD-1 mice were used to assess the viability of Cryptosporidium parvum oocysts after chemical disinfection. In vitro excystation and DAPI/PI staining provided similar estimates of viability in bench-scale experiments, but both of these methods significantly overestimated the viability when compared with infectivity (Pr < or = 0.01). Infectivity was the most reliable measure of the viability of C. parvum oocysts following chemical disinfection.
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Sidney Addelman, G. E. P. Box, W. G. Hunter et al. · Technometrics · 1979 · 6.5K citations
Andrew T. Campbell, Lucy J. Robertson, H.V. Smith · Applied and Environmental Microbiology · 1992 · 400 citations · Full text
Gordon R. Finch, E. Kathleen Black, Lyndon L. Gyürék et al. · Applied and Environmental Microbiology · 1993 · 138 citations · Full text