Production of a novel alkaline lipase by Fusarium globulosum using neem oil, and its applications

Abstract

Abstract A low-temperature, alkaline, and detergent-compatible lipase from Fusarium globulosum (FGL) has been produced using an inexpensive source, viz. neem oil. Maximum lipase production of 12 300 U/L was achieved in 96 h on optimizing various physicochemical parameters. The lipase exhibited remarkable stability in the presence of commercial detergents, bleaching agents, and proteases. The lipase showed a novel property of marked activation on prolonged incubation at alkaline pH. Wash performance analysis of the commercial detergent for removal of fatty stains improved upon addition of this lipase.