Normal values for some biochemical constituents in rabbits

Abstract

54 Belgian semi-lop rabbits from aclosed colony were used for the experiment. Environmental effects were minimised by employing a 12 h light cycle in a room thermostatically maintained at 17-] 8°C. The rabbits were equilibrated for 6 weeks before blood samples were taken when 12-14 weeks old and 2-2' 5 kg bodyweight. They were fed on standard commercial pelletted rabbit food (BOCM Silcock Ltd, Basing View, Basingstoke, RG21 2EQ) with anticoccidiosis supplement, based on Diet 18 (Bruce & Parkes, 1947). Food and water were allowed on demand but food was withheld from 1730 on Wednesdays. Samples were collected at 0900-1030 on Thursdays, immediately before cage cleaning. Cages were also cleaned on Monday mornings. Blood samples were collected from the marginal ear vein after dilatation with an infrared lamp, and treated with sequestrene anticoagulant (Turner-Stayne Products, York House, Empire Way, Wembley, HA9 OPS) and plain tubes. A single sample of 8-10 cm 3 was taken from each of the 54 rabbits. Labile component analysis was performed within 2 h and the serum separated. Analysis of other components was performed within 48 h of collection, the serum being stored at -20°C. All analyses were performed in duplicate and the mean taken. Estimations were controlled with commercial quality control serum as far as possible and the statistical parameters calculated with a computer (Table I).