<i>Vibrio</i> spp. Control at Brine Shrimp, <i>Artemia</i>, Hatching and Enrichment

Abstract

Abstract Experiments were conducted to evaluate different prophylactic methods to control the bacterial load in brine shrimp, Artemia , hatching. The first experiment evaluated three treatments to control Vibrio spp. during the Artemia hatching: microalgae ( Chaetoceros calcitrans ), probiotic ( Bacillus spp.), and antibiotic (Florfenicol). In the second experiment, Artemia metanauplius were enriched in distinct treatments with C. calcitrans , probiotic, and emulsion rich in docosahexaenoic and eicosapentaenoic fatty acids. Enriched Artemia metanauplius and nauplii (control) were offered to white shrimp, Litopenaeus vannamei , postlarvae ( PL 7 –PL 19 ). Presumptive Vibrio were quantified in Artemia , PL , and rearing water. Microalgae and probiotic were effective to control Vibrio spp. in Artemia nauplii. The enrichment process increased the Artemia bacterial load but did not affect Vibrio load in L . vannamei .