Genes & Development · 2002 · 107 citations · 25 references
Life SciencesGeneticsMolecular BiologyChromatin BiologyChromatin StructureDna ReplicationChromatinChromatin ArchitectureHeterochromatin OrganizationHistone ChaperonesHistone ProteinsNucleosome StructureChromatin Assembly FactorsChromatin-mediated Gene RegulationHistone Chaperone ComplexesHistone Chaperone FunctionHistone Chaperone InteractionsHistone Chaperones In Epigenetic InheritanceReplication-coupled Chromatin AssemblyReplication-coupled Nucleosome Organization
De novo chromatin assembly into regularly spaced nucleosomal arrays is essential for eukaryotic genome maintenance and inheritance. The Anti-Silencing Function 1 protein (ASF1) has been shown to be a histone chaperone, participating in DNA-replication-coupled nucleosome assembly. We show that mutations in the Drosophila asf1 gene derepress silencing at heterochromatin and that the ASF1 protein has a cell cycle-specific nuclear and cytoplasmic localization. Furthermore, using both genetic and biochemical methods, we demonstrate that ASF1 interacts with the Brahma (SWI/SNF) chromatin-remodelling complex. These findings suggest that ASF1 plays a crucial role in both chromatin assembly and SWI/SNF-mediated chromatin remodelling.
25
Crystal structure of the nucleosome core particle at 2.8 Å resolution
Karolin Luger, Armin W. Mäder, Robin K. Richmond et al. · Nature · 1997 · 9.4K citations
The RCAF complex mediates chromatin assembly during DNA replication and repair
Jessica K. Tyler, Christopher R. Adams, Shaw-Ree Chen et al. · Nature · 1999 · 547 citations