GENETIC CONTROL OF THE ANTIBODY RESPONSE TO POLY-<scp>L</scp>(TYR,GLU)-POLY-<scp>D,L</scp>-ALA--POLY-<scp>L</scp>-LYS IN C3H↔CWB TETRAPARENTAL MICE

Abstract

In order to further delineate the mechanisms underlying genetic unresponsiveness, tetraparental mice were constructed from immune response-1A gene high responder and low responder parental genotypes, then were immunized with poly-L-(Tyr,Glu)-poly-D,L-Ala--poly-L-Lys ((T,G)-A--L). An analysis of the total serum allotype mixture and of the antigen-binding capacity of the separated allotypes demonstrated that in the milieu of a tetraparental mouse, both high and low responder B cells could be stimulated equally to produce identical high titered anti-(T,G)-A--L responses. Furthermore, these studies show that effective stimulation could occur across a histocompatibility disparity.