Probing, Inhibition, and Crystallographic Characterization of Metallo‐β‐lactamase (IMP‐1) with Fluorescent Agents Containing Dansyl and Thiol Groups

Abstract

A series of fluorescent probes, N-(5-(dimethylamino)-1-naphthalenesulfonamido(alkyl)n)-3-thiopropionamide (DansylCnSHs), were rationally designed to detect and inhibit metallo-β-lactamase (IMP-1). These compounds were shown to function as fluorescent probes for and inhibitors of metallo-β-lactamases. The X-ray crystallographic structure shown indicates the potential of these agents for use as a new fluorescent probes for metallo-β-lactamases.