Proteose-Peptone Fraction of Bovine Milk: Lacteal Serum Components 5 and 8—Casein-Associated Glycoproteins

Abstract

Lacteal serum Components 5 and 8 were isolated from both heated and unheated skimmilk. At pH 8.6 Component 5 migrated as a double zone in starch-urea-gels (discontinuous buffer) and as a single zone in polyacrylamide gels (continuous buffer). Component 8 migrated in the ion front on starch gels but resolved into two principal zones in the polyacrylamide gel system, designated as 8-fast and 8-slow in ascending order of relative mobility. Components 8-fast and 8-slow were fractionated by gelpermeation chromatography on Bio-Gel P-10 polyacrylamide beads.