Long‐term culturing of plants with aseptic roots Determination of rape root exudates

Abstract

Abstract A plant culture system allowing growth of aseptic roots has been designed. One version of the system comprises vessels developed for plant root‐microorganism interaction studies in series. A second version has been built for measurements of different physiological parameters of the shoot and root system during growth periods of at least 2 months. The system was tested by determining soluble organic carbon glucose and sucrose in the root exudates of rape plants ( Brassica napus L.) grown for 3 weeks in the culture system. The plants were cultivated with a sterile or a non‐sterile root system, or with a root system infected with Verticillium dahliae Kleb.