Photolabile Protection for One‐Pot Sequential Native Chemical Ligation

Abstract

Newly developed phenacyl-type protection (Mapoc) for amines provides a facile and efficient synthetic protocol for peptides/proteins, in which sequential native chemical ligation (NCL) in the same reaction vessel can be conducted in a purification-free manner. A synthetic strategy of sequential NCL followed by oxidation with DMSO for disulfide-bond formation was successfully applied to the one-pot preparation of the human-brain natriuretic peptide (hBNP). Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2268/2005/z500272_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.