Diversity and Molecular Characterization of Dominant Bacillus amyloliquefaciens (JNU-001) Endophytic Bacterial Strains Isolated from Native Neem Varieties of Sanganer Region of Rajasthan

Abstract

Higher plants are ubiquitously colonized with bacterial endophytes that often lack readily detectable structures. In the present study seven novel endophytic bacterial species viz; Bacillus amyloliquefaciens (JNU-001), Burkholderia denitrificans (JNU-002), Pseudomonas aeruginosa (JNU-003), Xanthomonas campestris (JNU-004), Azotobacter tropicalis (JNU-005), Acetobacter xylinum (JNU-006) and Azospirillum lipoferum (JNU-007) has been recovered from native neem varieties at Sanganer areas namely Khonagorion, Muhana, Jagatpura, Goner, Watika, Kalwara and Bagru of Rajasthan state. In which Bacillus amyloliquefaciens was found dominant and associated endophytically in all the plants. Molecular characterization of the isolates was performed using 16S r-DNA restriction analysis. Similarity index in unweighted pair group method with arithmetic mean programme clustered the isolates according to their geographical distribution and confirmed the endophytic association between these novel bacterial strains and neem varieties.