Applied and Environmental Microbiology · 1981 · 145 citations · 14 references
Life SciencesBiochemistryMicrobiologyBiocatalysisEnzymologyMicrobial MetabolismAmino Acid FermentationAmino Acid MetabolismAmino Acid ProductionEnzyme KineticsMicrobial Enzyme ProductionPhenylpropanoid MetabolismEnzyme ActivityEssential Amino Acid MetabolismIsoleucine MetabolismL-isoleucine FermentationL-phenylalanine FermentationPhenylalanine Ammonia-lyase PathwayPhenylalanine BiosynthesisPhenylalanine Metabolism
An enzymatic method using l-phenylalanine ammonia-lyase (EC 4.3.1.5) for the rapid conversion of trans-cinnamic acid to l-phenylalanine has been investigated. With Rhodotorula glutinis, enzyme activity as high as 0.3 U/ml of culture broth was obtained. The enzyme activity was kept stable for a relatively long time during cultivation by the addition of l-isoleucine. Optimization of the parameters of the conversion reaction resulted in accumulation of 18 mg of l-phenylalanine per ml of reaction mixture. The conversion yield from trans-cinnamic acid was about 70%. The method may provide a rapid and practical way to produce l-phenylalanine useful as an essential amino acid.
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Immobilization of enzymes and microbial cells using carrageenan as matrix
Tetsuya Tosa, Tadashi Sato, Takao Mori et al. · Biotechnology and Bioengineering · 1979 · 157 citations