International Immunology · 1992 · 11 citations · 0 references
Life SciencesImmunologyMolecular BiologyCellular ImmunologyImmunogeneticsMolecular ImmunologyAlloantigen RecognitionAntigen RecognitionHuman Leukocyte Antigen SystemMajor Histocompatibility ComplexT Cell ActivationT Cell BiologyHla AllelesHla ImmunogeneticsHla-restricted T Cell RecognitionT Cell Receptor SequencingT Cell Receptor StructureT-cell Receptor ClonalityT-cell Receptor Gene LociT-cell Receptor Gene Usage
To investigate whether T cells recognizing the same HLA molecule may demonstrate homology in parts of their TCR, five different HLA-DQw8-specific T lymphocyte clones (TLC) were studied. The TCR alpha and -beta genes of four alloreactive, HLA-DQw8-specific and one antigen-specific TLC were sequenced. All TLC used different V alpha and V beta genes. However, four of the TLC shared a certain CDR1 beta motif and all five used either J beta 2.3 or -2.5. In addition, two used the same J alpha. The results indicate a possible preferential usage of certain TCR structures by T cells specific for DQw8.