Shieh medium supplemented with tobramycin for selective isolation of Flavobacterium columnare (Flexibacter columnaris) from diseased fish

Abstract

Tobramycin was found to be less inhibitory to Flavobacterium columnare (formerly Flexibacter columnaris) than to other fish-associated bacteria. The selective capacity of Shieh medium, an isolation medium described for this species, was markedly enhanced by adding tobramycin at a concentration of 1 microgram/ml.