Estimation of Total Cholesterol in Serum by a Micro Method

Abstract

Abstract A method has been described for the estimation of total cholesterol in 0.02ml. of serum without the need for micro apparatus. It involves the measurement of the fluorescence developed after the addition of sulfuric acid to a solution of cholesterol in a mixture of 1,1,2-trichloroethane and acetic anhydride(1). The results obtained with both human and rat sera have been in good agreement with those obtained with a standard colorimetric method(2), and the standard deviation for an estimation carried through in duplicate, owing to error within the method, has been ±4-5 mg. per 100 ml. under routine conditions.