Proceedings of the National Academy of Sciences · 1994 · 29 citations · 12 references
Life SciencesMolecular BiologyGene ExpressionProtein TranslationTranscriptionNucleic Acid SynthesisProtein SynthesisRna ModificationRna TranscriptionTranslational RegulationAntisense RnasCell-free Transcription-translation SystemsCell-free Translation SystemsChloramphenicol AcetyltransferaseConcentration-dependent BindingInitiation Codon RecognitionOligonucleotide Hybridization
Antisense oligonucleotides are ordinarily targeted to mRNA by double-stranded (Watson-Crick) base recognition but are seldom targeted by triple-stranded recognition. We report that certain all-purine methylphosphonate oligodeoxyribonucleotides (MPOs) form stable triple-stranded complexes with complementary (all-pyrimidine) RNA targets. Modified chloramphenicol acetyltransferase mRNA targets were prepared with complementary all-pyrimidine inserts (18-20 bp) located immediately 3' of the initiation codon. These modified chloramphenicol acetyltransferase mRNAs were used together with internal control (nontarget) mRNAs in a cell-free translation-arrest assay. Our data show that triple-strand-forming MPOs specifically inhibit protein synthesis in a concentration-dependent manner (> 90% at 1 microM). In addition, these MPOs specifically block reverse transcription in the region of their complementary polypyrimidine target sites.
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Douglas A. Melton, Paul A. Krieg, Michael R. Rebagliati et al. · Nucleic Acids Research · 1984 · 6.4K citations · Full text
Cynthia C. Smith, Laure Aurelian, M. P. Reddy et al. · Proceedings of the National Academy of Sciences · 1986 · 296 citations · Full text