High performance size exclusion chromatography of starch with dimethyl sulfoxide as the mobile phase

Abstract

Abstract Two types of high performance size exclusion chromatographic (SEC) columns were tested for the characterization of starch samples: μ Bondagel and Aquapore. The mobile phase was dimethyl sulfoxide (DMSO) containing 0.03 M sodium nitrate maintained at 80°C. The results indicated that both μ Bondagel and Aquapore can be used to determine the relative molecular weight of starch. The exclusion limits for these columns exceed ten million. Preliminary results using μ Styragel are also reported. Examples of SEC analysis of starch, including starch with anionic groups, are given.