Random Insertion of a TetR-Inducing Peptide Tag into <i>Escherichia coli</i> Proteins Allows Analysis of Protein Levels by Induction of Reporter Gene Expression

Abstract

The insertion element InsTipalpha was constructed to generate protein expression data. It randomly fuses the TetR-inducing peptide Tip to the affected reading frame. Fusion protein expression is quantified by Tet-regulated reporter gene expression. The expression patterns of tagged Escherichia coli genes fully agree with published data from transcriptional fusions or microarrays, validating the Tip tag approach.