The in vitro characterization of dextran-based nanobubbles as possible DNA transfection agents

Abstract

Nanobubbles consisting of a diethylaminoethyl-dextran (DEAE) shell and a perfluoropentane core were prepared as a novel DNA transfection agent. Nanobubble diameters were less than 500 nm, they possessed positive surface charge, and the ability to complex with and transfect DNA. DEAE nanobubbles present a novel platform for DNA delivery into cells that may be combined with ultrasound for imaging and targeting purposes.