Journal of Virology · 1997 · 21 citations · 26 references
Life SciencesMicrobiologyMolecular BiologyMolecular VirologyVaccinologyVirologyAnimal VirusesViral InfectionsViral StructureViral VaccinesViral Vector VaccinesVirus StructureReplication-competent VectorsRetroviral VectorsVaccinia Virus VectorsVector-borne Animal VirusesViral Envelope ProteinsViral Expression VectorsViral VectorsVirus Evolution Analysis
Semliki Forest virus vectors encoding murine leukemia virus (MLV) envelope protein with a truncated cytoplasmic tail generate submicrometer, cell-associated, membranous particles that transmit replication-competent vector RNA specifically to cells bearing the MLV receptor. Such "minimal" viruses could have applications as retroviral vaccines or in the study of virus evolution.
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Site-directed mutagenesis by overlap extension using the polymerase chain reaction
Steffan N. Ho, Henry D. Hunt, Robert M. Horton et al. · Gene · 1989 · 7.5K citations
Sindbis Virus: An Efficient, Broad Host Range Vector for Gene Expression in Animal Cells
Cheng Xiong, Robin Levis, Ping Shen et al. · Science · 1989 · 409 citations