Sequence Specific Label-Free DNA Sensing Using Film-Bulk-Acoustic-Resonators

Abstract

<para xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> A label-free biosensor (for detection of DNA sequences) based on film-bulk-acoustic-resonator (FBAR) is presented in this letter. The FBAR's resonant frequency shifts to a lower value when a complementary single-strand DNA sequence is hybridized with a DNA probe sequence on an Au-coated FBAR surface. The sensor is capable of distinguishing a complementary DNA that is mismatched to a probe DNA by a single nucleotide. The label-free, highly sensitive and selective, and real-time detection of DNA sequence could easily be made into an array for combinatory DNA sequencing, and could possibly help geneticists to detect specific DNA sequences accurately and fast, without any expensive optical scanning or imaging. </para>