A simple, rapid method of solubilizing algal tissue for scintillation counting1

Abstract

The Mahin‐Lofberg perchloric acid‐hydrogen peroxide method of solubilizing animal tissues is effective in digesting labeled Macrocystis and other seaweeds for liquid scintillation counting. The reaction is carried out in the scintillation vial and is faster and more convenient than extraction or combustion of samples. Solubilization is practically complete, counting efficiency is high, and recovery of 14 C is greater than 90%. Over 250 mg of fresh Macrocystis blade tissue can be digested per vial; dry samples or extracted residues can also be solubilized.