Molecular and Cellular Biology · 1986 · 108 citations · 7 references
Life SciencesBiochemistryMolecular BiologyCell SignalingCellular BiochemistryChemical KineticsBinding AffinityBinding KineticsLigand BindingLigand Binding KineticsMichaelis-menten KineticsProtein Interaction KineticsAssociation Rate ConstantsDissociation Rate ConstantsEquilibrium Binding ConstantEquilibrium Dissociation ConstantIsotope DilutionPheromone SignalingProtein-ligand Binding KineticsRate Constant Determination
The number of alpha-factor binding sites on yeast MATa cells (8,000) and the equilibrium dissociation constant (6 X 10(-9) M) were determined from direct binding experiments. These values correct our previously reported estimates (D. D. Jennes, A. C. Burkholder, and L. H. Hartwell, Cell 35:521-529, 1983) that were based on indirect isotope dilution studies, and they lead to a revised rate constant for the association process (kon = 3 X 10(5) mol-1 s-1).
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Numbers of Receptor Sites from Scatchard Graphs: Facts and Fantasies
Irving M. Klotz · Science · 1982 · 678 citations