Concepedia

Addition of an N-terminal epitope tag significantly increases the activity of plant fatty acid desaturases expressed in yeast cells

Jami B. O’Quin, Robert T. Mullen, John M. Dyer

Applied Microbiology and Biotechnology · 2009 · 17 citations · 26 references

DOI

Concepts

References

26

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Harry Towbin, T. Staehelin, J. Gordon · Proceedings of the National Academy of Sciences · 1979 · 53.8K citations · Full text

, , +13

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

E. G. Bligh, W. J. Dyer · Canadian Journal of Biochemistry and Physiology · 1959 · 48.9K citations

, , +9

Current protocols in molecular biology

Tom Sargent · Analytical Biochemistry · 1988 · 7.3K citations

, , +10

Eight Histidine Residues Are Catalytically Essential in a Membrane-Associated Iron Enzyme, Stearoyl-CoA Desaturase, and Are Conserved in Alkane Hydroxylase and Xylene Monooxygenase

John Shanklin, Edward Whittle, Brian G. Fox · Biochemistry · 1994 · 770 citations

, , +9

Preparation of Yeast RNA

Martine A. Collart, Salvatore Oliviero · Current Protocols in Molecular Biology · 1993 · 460 citations

, , +10