Isolation, partial sequencing, and phylogenetic analyses of<i>Soybean mosaic virus</i>(SMV) in Ontario and Quebec

Abstract

Soybean mosaic virus (SMV) can be present wherever soybean is grown, causing yield loss and seed quality deterioration. We have isolated SMV from eight soybean samples collected from fields in Ontario and Quebec, the two major soybean producing provinces in Canada. The coat protein region of these isolates was amplified, sequenced, and compared with 14 published sequences to determine the phylogenetic relationship among them. It was found that all eight isolates were most related to the SMV G2 group identified in the US. Further parsimony analysis of partial SMV sequences suggests that there are at least three SMV genotypes present in Ontario and two in Quebec. Three representative isolates were used for a pathogenicity test. As expected, all of them could infect a susceptible cultivar and a resistant cultivar carrying the resistance gene Rsv3, but could not infect resistant cultivars carrying the resistance genes Rsv1, Rsv1-h, or Rsv4