Phytopathology · 1994 · 215 citations · 0 references
AgricultureGeneticsPlant SciencesBreeding For Disease ResistanceMolecular BreedingPlant GeneticsGene MappingMolecular Markers In PlantsPlant Breeding GeneticsPlant Virus ResistanceResistance Gene DiscoveryResistant Crop VarietiesAnthocyanin ColorationAnthocyanin PigmentationMicrosatellite MappingPigmentationSimple Sequence Repeat AnalysisSimple Sequence RepeatsSoybean BreedingVirus-resistant Plants
Restriction fragment length polymorphisms (RFLPs) and microsatellites or simple sequence repeats (SSRs) were used as genetic markers to identify the chromosomal location of Rsv, a gene conferring resistance to soybean mosaic virus (SMV). An F 2 population was constructed from a cross between soybean line PI 96983 as the resistant parent and cultivar Lee 68 as the susceptible parent. Twenty-five RFLP and three SSR loci, polymorphic between the parental lines, were analyzed in 107 F 2 individuals. Genotypes of Rsv were determined by inoculating F 2.3 progeny RACT with the G1 strain of SMV. Data also were collected for an additional soybean gene (w1/W1), which controls anthocyanin pigmentation in hypocotyls and flowers [...]