β-Mannosynthase: Synthesis ofβ-Mannosides with a Mutantβ-Mannosidase

Abstract

Engineering enzymes: The glutamic acid nucleophile of a retaining β-mannosidase has been replaced with a serine residue to form a β-mannosynthase. When the new enzyme is provided with an α-mannosyl fluoride donor and an appropriate acceptor, β-mannoside linkages are synthesized (see scheme). Remarkably, α-mannosyl fluoride can be generated in situ by providing the mannosynthase with excess fluoride ion. Supporting information for this article is available on the WWW under http://www.angewandte.com or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.