Synthesis of 2‐Deoxy‐maltooligosaccharides with Phosphorylase and Their Degradation with Amylases

Abstract

Abstract By reaction of phosphorylase with D‐glucal and inorganic phosphate instead of the natural substrate α‐D‐glucosyl phosphate novel 2‐deoxy‐maltooligosaccharides could be synthesized. Their properties and characterization by NMR spectroscopy, mass spectrometry and HPLC are described. Under similar conditions, except a higher phosphate concentration, 2‐deoxy‐α‐D‐glucosyl phosphate is obtained. This can also serve for the enzymatic elongation of the primer with 2‐deoxy‐glucosyl residues. The unnatural 2‐deoxy‐maltooligomers could be applied successfully as substrates for the exo‐cleaving enzymes glucoamylase and β‐amylase.