Highly Efficient Biosynthesis of the Oligosaccharide Moiety of the GD3 Ganglioside by Using Metabolically Engineered <i>Escherichia coli</i>

Abstract

Express order for oligosaccharides: A microbiological process for the synthesis of the carbohydrate portion of gangliosides GD3 and GT3 is described (see structure). Lactose and sialic acid are used as exogenous precursors by a metabolically engineered Escherichia coli strain that overexpresses the bifunctional sialyltransferase cstII gene from Campylobacter jejuni. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2005/z461507_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.