Science · 1997 · 481 citations · 22 references
AgricultureGeneticsMolecular GeneticsInsect GeneticsPlant GeneticsPlant Molecular BiologyDisease ResistanceDisease Resistance GeneticsInsect ResistancePest ResistancePest Resistance GeneticsPlant Disease ResistanceCloningCyst Nematode ResistancePlant Disease Resistance GenesPlant Resistance GenesPlant Resistance To NematodesPositional CloningResistance Locus CharacterizationResistance To Nematode Disease
The Hs1(pro-1) locus confers resistance to the beet cyst nematode (Heterodera schachtii Schmidt), a major pest in the cultivation of sugar beet (Beta vulgaris L.). The Hs1(pro-1) gene was cloned with the use of genome-specific satellite markers and chromosomal break-point analysis. Expression of the corresponding complementary DNA in a susceptible sugar beet conferred resistance to infection with the beet cyst nematode. The native Hs1(pro-1) gene, expressed in roots, encodes a 282-amino acid protein with imperfect leucine-rich repeats and a putative membrane-spanning segment, features similar to those of disease resistance genes previously cloned from higher plants.
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A new method for predicting signal sequence cleavage sites
Gunnar von Heijne · Nucleic Acids Research · 1986 · 4.6K citations · Full text