Angewandte Chemie International Edition · 2002 · 148 citations · 35 references
EngineeringBiochemistryChemistryAnalytical ChemistryBiosensorsCatalysisBiocatalyst DiscoveryBiocatalyst ScreeningEnzyme AssaysEnzyme ScreeningEnzyme-linked Immunosorbent AssayImmunoassay AnalysisCatalytic Activity AssaysEnantioselective CatalysisEnantioselective DetectionEnantioselectivity ScreeningEnzyme Screening AssaysHigh-throughput Screening AssaysImmunoassay Drug ScreeningRegioselectivity Screening
More than 1000 yields and ee determinations are possible per day using an immunoassay. This highly efficient screening method is based on the remarkable binding specificity of antibodies. It has been employed in the development of straightforward procedures for the direct conversion of α-keto acids into chiral α-hydroxy acids, for which the enantioselective reduction of benzoyl formic acid to mandelic acid serves as a model reaction (see scheme).
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