High-Throughput Screening of Enantioselective Catalysts by Immunoassay

Abstract

More than 1000 yields and ee determinations are possible per day using an immunoassay. This highly efficient screening method is based on the remarkable binding specificity of antibodies. It has been employed in the development of straightforward procedures for the direct conversion of α-keto acids into chiral α-hydroxy acids, for which the enantioselective reduction of benzoyl formic acid to mandelic acid serves as a model reaction (see scheme). Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2002/z17920_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.