Random Mutagenesis of <i>Clostridium cellulolyticum</i> by Using a Tn <i>1545</i> Derivative

Abstract

Further understanding of the plant cell wall degradation system of Clostridium cellulolyticum and the possibility of metabolic engineering in this species highlight the need for a means of random mutagenesis. Here, we report the construction of a Tn1545-derived delivery tool which allows monocopy random insertion within the genome.