Selective Immobilization of Proteins onto Solid Supports through Split‐Intein‐Mediated Protein Trans‐Splicing

Abstract

Without a trace: The immobilization of biologically active proteins onto a solid support through split-intein-mediated protein trans-splicing reactions requires only very dilute samples. Not only is this process traceless but it does not require purification of the protein, thereby making possible the direct immobilization of proteins from complex mixtures such as cell lysates. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2006/z503475_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.