Solid‐phase synthesis of trypsin inhibitor CMTI III from squash seeds (<i>Cucurbita maxima</i>)*

Abstract

Using the solid‐phase procedure, a linear 29‐peptide with the reported sequence of trypsin inhibitor CMTI III was synthesized and oxidized to give three disulphide bridges. After affinity chromatography on an immobilized anhydrotrypsin column the synthetic product was shown by various physical techniques to be identical with the trypsin inhibitor CMTI III from squash seeds ( Cucurbita maxima ).