Publication | Closed Access
On the Hydrazinolysis of Proteins and Peptides: A Method for the Characterization of Carboxyl-terminal Amino Acids in Proteins
382
Citations
4
References
1952
Year
Carboxylterminal Amino AcidProtein ChemistryBiochemistryProtein FoldingCarboxyl-terminal Amino AcidsBioanalysisGlycobiologyMedicineNatural SciencesPeptide LibraryPeptide SynthesisPeptide EngineeringProtein EngineeringBrief MethodPharmacologyEnzymatic ModificationCarbohydrate-protein InteractionBiophysics
Abstract (1) A brief method for the characterization of carboxyl-terminal amino acids in peptides and proteins was investigated. (2) Several new amino acid hydrazides were synthesized. (3) Soveral di- and tri-peptides were hydrazinolyzed and identified their carboxylterminal amino acid. (4) The method was applied to beef insulin and tyrocidin for the demonstration of its applicability.
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