Elimination of <i>Lactobacillus plantarum, Corynebacterium</i> spp., <i>Staphylococcus saprophyticus</i> and <i>Pseudomonas paucimobillis</i> from micropropagated <i>Hemerocallis, Choisya</i> and <i>Delphinium</i> cultures using antibiotics

Abstract

C. LEIFERT, H. CAMOTTA, S.M. WRIGHT, B. WAITES, V.A. CHEYNE &amp; W.M. WAITES. 1991. The antibiotic sensitivity patterns of bacterial contaminants isolated from micropropagated plant cultures were determined. Lactobacillus plantarum, Corynebacterium spp., Staphylococcus saprophyticus and Pseudomonas paucimobilis were eliminated from plant tissue cultures of Hemerocallis, Choisya and Delphinium by incorporating combinations of antibiotics into the plant growth medium. Elimination of contaminants resulted in increased growth rates of all plant cultures, except Choisya infected with Staph. saprophyticus. Pseudomonas maltophilia was not eliminated from Delphinium by any of the antibiotic treatments tested.