Protein synthesis by pure translation systems

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

Donald B. Smith, Kevin S. Johnson · Gene · 1988 · 6K citations

Life Sciences, Biochemistry, Molecular Biology +12

Cell-free translation reconstituted with purified components

Yoshihiro Shimizu, Akio Inoue, Yukihide Tomari et al. · Nature Biotechnology · 2001 · 2K citations

Life Sciences, Biochemistry, Molecular Biology +9

A General Method for Site-specific Incorporation of Unnatural Amino Acids into Proteins

Christopher J. Noren, Spencer J. Anthony‐Cahill, Michael Griffith et al. · Science · 1989 · 1K citations

Life Sciences, Biochemistry, Molecular Biology +14

In vitro virus: Bonding of mRNA bearing puromycin at the 3′‐terminal end to the C‐terminal end of its encoded protein on the ribosome in vitro

Naoki Nemoto, Etsuko Miyamoto‐Sato, Yuzuru Husimi et al. · FEBS Letters · 1997 · 458 citations

Life Sciences, Microbiology, Molecular Biology +16

Ribosome display efficiently selects and evolves high-affinity antibodies <i>in vitro</i> from immune libraries

Jozef Hanes, Lutz Jermutus, Susanne Weber-Bornhauser et al. · Proceedings of the National Academy of Sciences · 1998 · 289 citations

Life Sciences, Biochemistry, Molecular Biology +15