Bioconjugate Chemistry · 2007 · 22 citations · 7 references
Natural SciencesBiochemistryMolecular BiologyBioorthogonal ChemistryNucleic Acid BiochemistryOrganic ChemistryChromatographic PurificationChromatographyFluorescence SpectroscopyOligonucleotide ChemistrySpectroscopy Of BiomoleculesChemical Modification Of OligonucleotidesColumn ChromatographyFluorescence Emission SpectraOligonucleotide SynthesisOrganic FluorophoresPhosphoramidate OligonucleotidesPhosphoramidite ChemistryProtecting Group Chemistry
Pentafluorophenyl esters of 5- and 6-carboxyfluorescein-3',6'-O-dipivalate can be easily separated in multigram quantities by column chromatography. The individual isomers were converted into stable phosphoramidites suitable for oligonucleotide synthesis. The use of the cyclohexylcarbonyl (Chc) protecting group instead of pivaloyl (Piv) facilitates the separation of isomers. The fluorescence spectra of 5- and 6-carboxyfluoresceins on oligonucleotides were compared.
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Fluorescent dye phosphoramidite labelling of oligonucleotides
Pete Theisen, Christie McCullum, Krishna G. Upadhya et al. · Tetrahedron Letters · 1992 · 59 citations